Abstract: In order to understand the contaminations status of main heavy metals in farmland soil from mine area, the chemical forms and biological effect of heavy metal Cu, Zn and Pb in farmland soil from an exploiting coal mine region in Henan Province were analyzed through field investigation and laboratory analysis in the manuscript. The results showed that: the content of heavy metal Zn was larger than that of Cu and Pb in soil. The mine exploitation didn’t cause the obvious pollution of these metals according to Grade Ⅲ criteria of the national soil environmental quality standard (GB15618-1995). To the exchangeable form (EXC), carbonate bound form (CAB), Fe-Mn oxide bound form (FMO), organic matter bound form (OM), and residue form (RES) of 5 chemical forms by Tessier (1979) for each element in soil, RES was of the highest proportions. The average value of bioavailability coefficients for Cu, Zn and Pb were respectively 0.09, 0.05 and 0.16 based the ratio of both EXC and CAB to the total amount, which indicated the order of bioavailability was Pb > Cu > Zn. The average value of transport coefficient for Cu and Zn was respectively 0.026 and 0.020 while that for Pb was 0.083 based the ratio of EXC to the total amount, which revealed the transport ability for heavy metals was Pb > Cu > Zn. It was to help determine the biological effect of Pb was the highest.