Abstract: To make clear the pathogens of Root Rot disease of Polygonatum odoratum (Mill.) Druce., diseased rots of Polygonatum odoratum cultivated in Xinshao of Hunan Polygonatum odoratum (Mill.) Druce. base were collected using for isolation and identification of pathogens of Root Rot .13 antifungal Chinese herbal extracts were first used to test with the pathogens to screen for the best one by using the growth rate method. The result showed that the pathogen was Fusarium solani; extracts from Artemisiae argyi Levl.et Vant.,Sophorae flavescentis Ait.,Fructus Evodiae, Angelica sinensis (olive.) diels, showed obvious effects to inhibit mycelial growth. The results provided dates for the disease control of Polygonatum odoratum (Mill.) Druce.