Abstract: The objective of this study was to investigate the optimum amount of fertilization of ‘Qinyan 96’ under the treatments of 3 factors (N, P and K), 4 experimental designs (without nitrogen, reducing nitrogen, normal, and more fertilizer), the content of nitrogen, phosphorus and potassium and nutrient absorption efficiency was measured under the different proportion of fertilizer at rosette and mature stage. The results showed that: the nutrient content of tobacco leaf was increased with the augment of nitrogen applying at rosette stage, while the content of nitrogen in leaf was decreased with the increment of phosphorus applying, phosphorus content did not change significantly, potassium content increased first and then decreased. Nitrogen, phosphorus and potassium content of tobacco leaf was higher when the amount of nitrogen, phosphorus, potassium fertilizer was 45, 30,90kg/hm2, respectively. In the maturity stage, N content of tobacco leaf was 2.57% and increased by 25.6%, compared with the control, when the amount of nitrogen, phosphorus, potassium fertilizer was 90, 60, 90 kg/hm2, respectively. When the amount of nitrogen, phosphorus, potassium fertilizer was 45, 90, 90 kg/hm2, P content of tobacco leaf was 1.30% and increased by 12.1%, when compared with the control. K content of tobacco leaf increased by 52.3%, when the amount of nitrogen, phosphorus, potassium fertilizer was 45, 60, 90 kg/hm2, respectively. N use efficiency of tobacco was the highest and increased by 6.76%. The suitable amount of nitrogen, phosphorus, potassium application was 45, 60, 90 kg/hm2 under this investigation of ‘Qinyan 96’.