Abstract: In order to optimize cultivating technologies for soybean production in super early type of soybean varieties, effects of nitrogen level on source-sink relationship and yield and quality were researched in present paper. By setting different nitrogen application rate, measured changes in different growth stages sources and libraries of various organs of the ultra - early maturing soybean varieties ‘Heihe 44’. The main results showed that, as nitrogen usage increased, the yield and protein content were improved and oil content descended. Added nitrogen usage doze accelerate accumulation of fresh and dried leaves weight and lessen the rate of aging and falling up of leaf. Under higher nitrogen usage, dried weight of stem were increased in the whole growth period and fresh stem weight increased at flowering and pod bearing stage and descended at seedling stage. Dried root weight accumulation were slowed by added nitrogen usage in whole growth period, fresh root weight accumulation were accelerated at stage of flower bud differentiation and aging rate were lowered at seed filling period and full ripe stage. Fresh pod wall weight increased and dried pod wall decreased as the nitrogen dosage increased. Fresh and dried seed weight increased by added nitrogen dosage.