Abstract: Ubiquitination extensively regulates the eukaryotic cell life activities. Ubiquitin E3 ligases play a specific role to recognize the targets for degradation via the ubiquitin-proteasome degradation system. This review summarizes the roles of E3 on hormone signaling, growth and development, abiotic and biotic stresses in rice (Oryza Sativa L.). The future research should focus on the dissection of the interaction between rice E3s and their targets and develop new strategies and advice for the improvement of rice quality, abiotc and biotic stress tolerance.