Abstract: In order to estimate scientifically land eco-security of ecologically fragile area and it’s change of cultivated land resources in Qinghai Province, an index system and the corresponding weight values were established based on “pressure-state-response” model and entropy weight method, and an integrated evaluation model was constructed with matter element model to evaluate land eco-security of Qinghai Province. Land eco-security change situation was analyzed in Qinghai Province during 2000 to 2010. Results indicated that, the whole level of land eco-security in Qinghai Province improved slightly from relative insecurity to critical security; The change of cultivated land eco-security index was different of each city/state in Qinghai Province and the distinction of different city/state were obvious. The main restriction factors of land eco-security was density of economy, cultivated land area per capita, forest and grass coverage ratio, proportion of natural disaster area and dosage of farmland fertilizer.