Abstract: In order to study the elimination of oxytetracycline in Pelteobagrus fulvidraco muscle. At 25℃ water temperature, following oral administration at a dose of 100 mg/kg of OTC per day for 5 days, the fish were sampled at certain time, and the concentration were determined by high performance liquid chromatography (HPLC). The average recovery rate of OTC in P. fulvidraco muscle was 80.2%, and the limit of detection was 0.05 μg/g. The results revealed that the elimination of OTC from muscle of P. fulvidraco was very slow, the withdrawal period of OTC in the muscle of P. fulvidraco was 46 days until the concentration of OTC reducing to 0.1 μg/g under this experiment condition.