Abstract: Allelopathic effects of aqueous extracts from root, stem and spikes of wheat straw on the seed germination and seedling growth of goosegrass (Eleusine indica) were tested with a petri dish bioassay method. The results indicated that they all inhibited the germination of goosegrass, germination rate and index decreased with the increasing concentration, and the treatment of 100mg?mL-1 had the strongest inhibition effect. Germination rate of root, stem and spikes aqueous extracts was 71.3%, 68.7% and 58.7% respectively, while the control was 84.5%, and germination index was 12.82, 6.77, 6.07 respectively, while the control was 18.39. Stem and spikes aqueous extracts inhibited the shoot length of goosegrass significantly only at the highest concentration, while root aqueous extracts had little effects. The root, stem and spikes aqueous extracts promoted the root growth of goosegrass at low concentrations but inhibited it at high concentrations. Compared with control, the root length increased by 17.1%，17.8% and 6.6% respectively when the concentration of root, stem and spikes aqueous extracts was 6.25mg?mL-1, but decreased by 25.6%、31.6% and 95.6% respectively when the concentration was 100mg?mL-1. EC50 was calculated through dose-response equation between different concentrations of root, stem and spikes aqueous extracts and root lengths, and the value was 219.182，157.545 and 41.007mg?mL-1 respectively