Abstract: The research was conducted to study the effects of different phosphate concentration on germination and seedling growth of perennial ryegrass (Lolium perenne L.). The experiment was designed as five phosphate concentration levels that 0(as CK), 25, 50, 75 and 100 mmol/L. Counted the burgeon seed number every day, and measured the seedling’s plumule and root length at the seventh day. Results showed that the perennial ryegrass seed germination energy, germination ratio, index of injury and vigor are higher at the level of 25 mmol/L than 0 mmol/L, but the seeds germination injury was significant inhibition(P<0.05) with the rise of the phosphate concentration, the higher phosphate concentration was not good for seed germination. The seedling’s plumule and root length were shorter under the 25 mmol/L than CK. And the seedling injury was significant enhanced(P<0.05) with phosphate concentration increase, the root was more serious than bud under the same density. Collectively, perennial ryegrass seed had a better germination performance at 25 mmol/L phosphate concentration, and had a less inhibition to the bud and root.