Abstract: To solve the problem of organic pollutants product in groundwater during exploitation and utilization of oil shale, the article took the oil degrading strains after domestication and cultivation to research the bio-degradation of the organic pollutants. The indoor research conducted at low temperature (10℃) and at shaking speed of 120 r/min. The experimental results show that: when the initial concentration of 200 mg/L, the optimum condition of the strains using the organic pollutants in groundwater was at pH 7, for inoculation of 5 mL, used ammonium chloride(0.05%) as the best nitrogen source and potassium sulfate(0.05%) as the best source of phosphorus. After 6 days at the optimum condition the concentration of total petroleum hydrocarbon could reduce from 200 mg/L to 33.94 mg/L. By the strains the degradation rate of the total petroleum hydrocarbon and COD was 83.03% and 67.58%, respectively. The biodegradation was proved to be first-order kinetic equation by kinetic analysis of degradation process under the initial concentration of the total petroleum hydrocarbon at 20 mg/L, and the total petroleum hydrocarbon initial concentration between 50-300 mg/L degradation meet zero-order kinetic equation.