Abstract: In order to elucidate the drought resistance of new strain alfalfa of ‘Zaxuan No.1’, the relative content of different varieties of alfalfa leaf angle, the relative content of chlorophyll(SPAD), relative permeability of cell membrane, peroxidase (POD), catalase (CAT), proline content and water saturation deficit (WSD) were determined in this paper. Based on clustering analytical method, the drought resistances of different alfalfa varieties were carried out. The experiment results showed that drought-resistance of alfalfa varieties order were new strain alfalfa of ‘Zaxuan No.1’> ‘Juren’> ‘Longzhong’> ‘Zhonglan No.1’> ‘Duputi’> ‘Tumu’ > ‘Aiji’.