Abstract: In order to provide evidence for resistance breeding scheme, the genetic of resistant to tobacco bacterial wilt were analyzed. The 15 tobacco germplasm including different resistant to bacterial wilt and its F1 cross by diallel mating design were identified in disease field of Anhui Province in China in 2010 and 2011. The results showed that: the resistance additive effect to tobacco bacterial wilt was dominant, the dominant effect was weak, additive×additive epistatic effect and G×E interaction effect was detected. The heritability in narrow sense of resistance was among 30.40%-57.14%.