Abstract: The objective of current study was to investigate the effects of soil layer replacement on soil enzyme and soil nutrient content of different layer. The catalase, urease and invertase of soil at seeding stage, flowering stage and mature stage of soybean were determined by conventional method, and the soil nutrient content was determined at mature stage of soybean by conventional method. The results showed that soil layer replacement changed the structure of soil, and had effect on the soil enzyme. The soil enzyme activity had significant differences between the different soil layer of soil layer replacement area and the control area. The soil nutrient content at soil layer replacement area showed that different change trend with the depth of soil increased, and there were significant differences compared with the control area. The soil layer replacement make against the soil nutrient content uniform distribution in different soil layers. The available phosphorus and available potassium of the control area soil showed that downward trend with the depth of soil increased. The soybean yield of soil layer replacement was 2245.5 kg/hm2, it was higher 20.1% than contrast. The soil layer replacement could overall activate soil nutrient and increased soil enzyme activity and increased soil active layer, improved the soil water supply capacity. The soil layer replacement has main effect to enhanced soil resilience and increased the yield of corp.