Abstract: The pSAD transit peptide gene, Cytochrome b5 gene and eGFP gene are cloned by PCR technique.Nuclear expression vectors were constructed based on the vectors pMD18-T,pET-28a and pDBle,cyt b5 gene and the fusion genes bG and TbG were inserted into pDBle.The recombinant plasmid pDBle-b5,pDBle-bG and pDBle-TbG were checked by restriction enzyme analysis,PCR and nucleic acid sequencing.The vectors were transferred into Chlamydomonas Reinhardtii(CW15) by glass beads and the transformants were identified with PCR.The transgenic Chlamydomonas were obtained after selecting with zeocin and they were confirmed positive by PCR amplification.PCR analysis of the genomic DNA from transgenic Chlamydomonas showed that the recombinant plasmids have integrated into Chlamydomonas Reinhardtii(CW15) genome.