Major Histocompatibility Complex IIB Gene Polymorphism and Its Association with Resistance/Susceptibility to Streptococcus agalactiae in Nile Tilapia (Oreochromis niloticus)

doi:10.11924/j.issn.1000-6850.2013-1164

Abstract

Abstract: In order to understand the relationship between polymorphism of the major histocompatibility complex genes and the disease resistance of Nile tilapia, the MHC IIB gene fragment was amplified from 40 susceptible individuals and 40 resistant individuals, using primer pairs of 2b-snp-sf and 2b-snp-sr. The length of the PCR products was 775 to797 bp. Among the PCR products of 775 to 797 bp nucleotides acid, 265 (33.2%) nucleotide positions were variable in resistant individuals and 255 (31.9%) in susceptible individuals, respectively. Among the putative 101 amino acid sequence encoded by the amplification product, 32 amino acid positions were polymorphic in resistant individuals and 33 in susceptible individuals, respectively. 7 different MHC IIB alleles which encoded 7 different amino acid sequences were identified among 441 clones from 80 individuals. Most alleles were commonly found in both individuals. Alleles Orni-DAB*0201 and Orni-DAB*0401 were significantly more prevalent in resistant individuals than in susceptible individuals, with 15.5%, 5.6% in resistant stock and 7.4%, 1.8% in susceptible stock respectively. Allele Orni-DAB*0501 and Orni-DAB*0601 were only found in resistant individuals. 70% in resistant individuals presented 2-4 alleles, only 30% individuals presented 1 allele. Whereas 42.5% in susceptible individuals presented 2-3 alleles, 57.5% individuals presented 1 allele. These results indicated that individuals possessed more MHC IIB alleles have stronger resistance ability to streptococcus disease. This study has identified potential candidate genes for further investigation into the breeding of disease-resistant strain of Nile tilapia.

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