Bioinformatics Analysis of Dihydroflavonol4-reductase and the Gene in Three Triticeae Crops

doi:10.11924/j.issn.1000-6850.2013-1225

Abstract

Abstract: To study the pigment synthesis’s related enzymes properties and genes of the color wheat, the dihydroflavonol-4-reductase genes of common wheat, blue-grained wheat, and quack grass were analyzed. The composition of amino acid sequences, molecular structure, physicaland chemical characters, subcellular localization, transmembrane helices, and molecular phylogenetic evolution, secondary and tertiary structure from NCBI were analyzed and predicted by bioinformatics software. The results demonstrated that, the length of three DFR genes were all 1.0 kb, which coded 354 aa and had a high homology amino acid, and they were all acidic, hydrophilic, stable protein, have no signal peptide and across membrane structure. Secondary structures of the protein were most of alpha helix and random coil. In the evolutionary tree could be seen the blue-grained wheat and common wheat genetic relationship were close. The result implied the enzymes of DFR in the triticeae crops’ properties was the same and had a conservative evolution. This study provided the basis of research the enzymes and their genes in further step.

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