Abstract: A field experiment was carried out in Fuquan City, Guizhou Province in 2012, to study the effects of the different times of top-dressing in the same amounts of fertilizer on yield and quality of organic flue-cured tobacco. The results showed that: there was no difference of growth period in different treatments. Top-dressing four times were significantly higher than the two times in agronomic traits, and grew best. As the increase of the times of fertilizer, it was favorable to raise the chlorophyll content and net photosynthetic rate. Top-dressing four times can increase the yield, production value, average price of flue-cured tobacco and ratio of first-class tobacco leaf, and the compositions of various compounds and their proportions were found suitable. With the increase in times of top-dressing, would help shorten the field growth period of the tobacco plant, agronomic traits to improve, increased photosynthetic indicators, to improve the quality of production.