Abstract: Study on the cut tobacco structure of traditional cutting, 30mm fixed-length cutting and 40mm fixed-length cutting, the changes of cut tobacco structure at the different sample points and the cigarette-making quality on different cutting mode were investigated. The results showed that: 1) comparing with traditional cutting, the whole cut rate and middle strands of 40mm fixed-length cutting were unchanged , while the whole cut rate of 30mm fixed-length cutting was decreased and middle strands was increased during the link of cut tobacco drying and cut-tobacco storage; 2) comparing with traditional cutting and 30mm fixed-length cutting, the average of loss of cigarette of 40mm fixed-length cutting reduced to a certain degree. Moreover, the qualification rate of loss of tobacco from the ends was higher; 3) comparing with traditional cutting, the standard deviation of individual cigarette weight of 40mm fixed-length cutting reduced by 9%,and the standard deviations of draw resistance and circumference also reduced to a certain degree. Conclusively, 40mm fixed-length cutting significantly increased the stability of physical quality of cigarette.