Abstract: The objective of this study was to find the optimum matching of hormones, water temperature and larval rearing weaning food. The methods were injected different hormones at different temperature during re-production and feeding different weaning food during seeding rearing. As studies have demonstrated that the fecundity of female Misgurnus anguillicaudatus was proportional to the sexual maturity, i.e. the higher sexual maturity, the more fecundity. For female Misgurnus anguillicaudatus which had a mature coefficient of 24%, the value of which absolute fecundity and relative fecundity of were 62142 and 990, respectively. But that of a mature coefficient of 9% were only 18207 and 367, respectively. The result indicated that different hormones had different effects towards Misgurnus anguillicaudatus’re-production, the effect of LRH-A2 was better compared to HCG, the fertilization rate of which were 83.04% and 78.84, respectively. The effect of spawning effect could improve which fertilization rate could reach 89.17% if those two hormones were mixed according to a certain proportion (LRH-A2 5μg per fish, HCG 100 IU per fish).When the temperature was increased, the spawning effect became better. The optimum water temperature of Misgurnus anguillicaudatus’ re-production ranged between (30±0.5)℃, the effect time of 6-8 h, induced spawning rate of 93.33% , fertilization rate of 89.26%. Weaning food was a very crucial factor during the cultivating of the Misgurnusanguillicaudatus. The result showed that larva survival rate could reach the highest point which was 93.3% when used rotifers as the weaning food, anemia nauplius, microparticle diets, egg yolk. Reasonable arrangement of weaning food could promote the growth of larval fish, as a result, the growth rate of larval conducted by a combination of egg yolk and rotifers was much higher than other three groups, which was 1.21, 1.04, 1.11 times faster than that of egg yolk, rotifers, anemia nauplius, respectively.