Abstract: In order to screened to excellent tobacco germplasm resources for tobacco breeding, and breeding seed storage in the National Gene Bank. 80 air-cured tobacco germplasm resources were evaluated for their character in the field. The result showed that there were large differences in the growing period duration, morphological and main agricultural character, and the CV of the budding period duration, main agricultural character was from 9.1% to 21.8% , 9.7% to 35.1% respectively. CV range from biger to smaller was the growing period duration, flowing duration, and the full growing period duration, node interval, stipe, angel between stem and leaf, plant height, effective leaf number, leaf width, stem girth, corolla diameter, calyx length, leaf length, angel between main vein and lateral veins, corolla length. The budding dates of‘Yongshengbianbianyan’was the earliest with 20 d, and than‘Luxiyamomi’and‘Mongbangshaiyan’, were 28 d and‘Pipaiyan’,‘Hekuotuyan’and‘Tuyan No.2’budding the latest with 52 d after being transplanting. The test screen out a few resources had less than 10 disease indexes, such as‘Xiangduoyan’,‘Luxichangyeyan’,‘Bayan’,‘Pipaiyan’,‘Shengnongdaye’,‘Tangpongyan’,‘Mangmongding No.4’,‘Xiachuengyan’,‘Niliangliuyeyan’, etc. With the help of clustering analysis, the germplasm resources were devided into two groups, and each group was devided into two subfamilies, and the each subfamily still could be devided into two types. Finally, the full date of each the germplasm resources were submitted to China Tobacco Heredity Breeding (North) Research Center to accomplish the bulild-up of China Tobacco Germplasm Resources Information Share Flatform, and 5 g seed of each germplasm resource was sent to for Chinese Tobacco Germplasm Bank.