Abstract: Mon-factor Randomized Complete Block Design (RCBD) experiment was conducted to analyze the effect of different functional leaf on the characteristics of grain-filling in malting barley cultivar (‘Ganpi7’). Variance analysis on yield and qualities based on the fitting result of grain-filling process via Logistic Growth Equation (LGE). The results indicated that grain-filling of malting barley cultivar best fits LGE under different treatments of functional leaf, with an S-like breakthrough curve of slow-fast-slow trend. The levels of different functional leaf influence 12 grain-filling parameters and malting qualities of malting barley, with both affected parameters and degree of effect varying with treatment. Effect of different treatments on screening rate and 1000-grain weight was significant and the change between them was apparently positive. The T4 treatment was significant different than others (screening rate: 72.17%, 1000-grain weight: 42.37 g). In order to breed hi-yield and high-quality malting barley, we should consider morphology index comprehensively in practice.