Abstract: In order to establish rapid propagation system of Vanda ‘Miss Joaquim’ ，effects of exogenous hormone and media on plantlets regeneration were studied. The results showed that: Decontamiated-explants can be obtained by inducing from floral stalk，and the sixty-five percent of plantlets formation ratio can be achieved on 1/2MS media supplemented with NAA0.5mg/L+6-BA2.0mg/L.There were different formation in different location in flora stalk buds and the toppest plantlets formation ratio were reached 80% in the third flora stalk buds；The propagation ratio was reached 5.3 on 1/2MS media supplemented with NAA0.2 mg/L+6-BA3.0 mg/L and the media with low concentration nutrient elements was benefit to the plantlets forming；With the length of 1~2cm, plantlets can be transplant into 1/2MS media to reduce roots ，and the percent s of survivor can be reached 99% when plantlets with roots were planted into greenhouse .