Abstract: In order to effectively protect the Chongqong wild lily resources development and utilization. It was studied the plant regeneration technology with Chongqing of wild lily buds as study material and using plant tissue culture as induce methods. The result indicated, the plant regeneration technology to the upcoming opening of lily flower buds as explants, disinfection and easy, no pollution; the organ bud (corolla, ovary, filaments, anther, style, column head, pedicel) inducing culture, showed that the test of No.1 culture medium of MS+ 6-BA 1.0 mg/L+ NAA 0.5 mg/L could induce corolla, ovary, filaments sprouting, No.2 of the medium MS+ 6-BA 0.5 mg/L+ NAA 0.5 mg/L can only induce corolla budding; bud proliferation coefficient MS+ 6-BA 1.0 mg/L+ NAA 0.5 mg/L was the highest, 3.2, robust growth and proliferation of seedling; rooting culture with No.14 (MS+ NAA 0.4 mg/L) induction of lily root, rooting rate was 100%; transplanting results in No.18 (MS+ IBA 0.5 mg/L) on the rooting seedling survival rate was higher, reaching 85%.