Extraction of Polysaccharide from Perilla frutescens Seed with Microwave and Its Hepatoprotective Activity

doi:10.11924/j.issn.1000-6850.2013-1914

Abstract

Abstract: In order to select the best extraction method and the hepatoprotective activity of Perilla frutescens, Perilla frutescens seeds were smashed and defatted with petroleum ether, then were studied the effect of the solvent/material ratio, microwave power and extracting time on the extraction rate. The hepatoprotective activity of Perilla frutescens seeds polysaccharides was studied. The results showed that the optimal extracting conditions were followed by a solvent and material ratio of 25:1, with microwave power at 480 W for 2 min, and the extraction rate was 9.06% . Perilla frutescens seeds polysaccharides had significant effect on decreasing serum levels of AST and ALT in mice (P<0.01). And the liver color and elasticity of polysaccharides treated mice were better than the control group with only CCl4 treated, and the spotty necrosis was significantly less, the liver coefficient and spleen coefficient were decreased (P<0.05). These results indicated that the Perilla fr utescens seeds polysaccharides had hepatoprotective activity on CCl4 induced liver injured mice. This study provided an important foundation for the research and development of Perilla frutescens polysaccharides on hepatoprotective drugs.

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