ISSR Analysis on Genetic Diversity of Coconut (Cocos nucifera L.) Germplasm Resources in Yunnan

doi:10.11924/j.issn.1000-6850.2013-1964

Abstract

Abstract: In order to reveal genetic diversity of coconut (Cocos nucifera L.) germplasm resources in Yunnan and promote their effective utilization, 60 accessions of coconut germplasm collected from different locations in Yunnan were used as experimental materials and the ISSR molecular marker technique was employed to analyze their genetic diversity. The result showed that 272 loci were amplified from the accessions by means of 27 ISSR primers, and 230 loci were polymorphic, the percentage of polymorphic loci was 84.56%. PIC index of the 27 primers was 0.85. SM similarity coefficient averaged out at 0.664. According to the SM similarity coefficient, a dendrogram for the accessions was obtained by using UPGMA method of the software NTSYSpc2.10e, and the 60 accessions were clustered into 3 groups (A, B and C). Among them, group A was composed entirely of local germplasm separated from others, group C included not only some of the local germplasm and but also others from other places. The results revealed that there were rich genetic diversity among the coconut germplasm resources in Yunnan, and suggested the relation of independence and relevance all existed in coconut germplasm in Yunnan and other places. These results also would provide a reference for further research and development of coconut germplasm resources in Yunnan.

References

[1] 沈雁,周焕起,唐龙祥.国际椰子种质资源研究与利用概况[J].河北农业科学,2009,13(3):13-14.
[2] 冯美利,李杰,王必尊.我国椰子综合研究进展概述[J].中国热带农业,2007(5):30-31
[3] 陈良秋,万玲.中国椰子史略[J].现代农业科技,2007(15):222-223.
[4] 毛彧.海南椰子种质资源经济研究[D].海口:海南大学,2011:25-26.
[5] Upadhyay A, Jayadev K, Manimekalai R, et al. Genetic relationship and diversity in Indian coconut accessions based on RAPD markers [J]. Scientia Horticulturae,2004,99:353-362.
[6] Meerow A W, Wisser R J, Brown J S, et al. Analysis of genetic diversity and population structure with Florida coconut (Cocos nucifera L.) germplasm using microstellite DNA with special emphaiss on the Fiji Dwarf cultivar[J]. Theor Appl Genet,2003,106: 715-726.
[7] Teulat B, Aldam C, Trehin R, et al. An analysis of genetic diversity in coconut (Cocos nucifera L.) populations from across the geographic range using sequence-tagged microsatellites (SSRs) and AFLPs[J]. Theor Appl Genet,2000,100:764-771.
[8] Manimekalai R. Inter simple sequence repeat (ISSR) markers for identification of germplasm and diversity analysis in coconut (Cocos nucifera L.) [J]. International Journal of Innovative Horticulture,2012,1(1):63-67.
[9] 潘坤,王文泉,唐黎黎,等.椰子 ISSR遗传多样性分析[J].果树学报, 2010,27(2):238-243.
[10] Herrán A, Estioko L, Becker D, et al. Linkage mapping and QTL analysis in coconut (Cocos nucifera L.)[J]. Theor Appl Genet,2000, 101:292-300.
[11] Baudouin L, Lebrun P, Konan J L, et al. QTL analysis of fruit components in the progeny of a Rennell Island Tall coconut (Cocos nucifera L.) individual[J]. Theor Appl Genet ,2006,112:258-268.
[12] Botstein D, White R L, Skolnick M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. Am J Hum Genet. 1980,32(3):314-331.
[13] Daniel P, Gao F Y, Giovanna A. Inter-simple sequence repeat markers for fingerprinting and determining genetic relationships of walnut (Juglans regia) cultivars[J]. Amer Soc Hort Sci,2002,127(1): 75-81.
[14] Gupta M, Chyi Y S, Romero S J. Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats[J]. Theor Appl Genet,1994,89:998-1006.
[15] Blair M W, Panaud O M C, Couch S R. Inter-simple sequence repeat (ISSR) amplification for analysis of microsatellite motif sequence and fingerprinting in rice (Oryza sativa L.) [J]. Theor Appl Genet,1999,98:780-792.
[16] Ratnaparkhe M B, Santra D K, Tullu A. Inter-simple sequence repeat polymorphisms and linkage with a fusarium wilt resistance gene in chickpea[J]. Theor Appl Genet,1998,97:515-519
[17] 钱韦,葛颂,洪德元.采用 RAPD和 ISSR标记探讨中国疣粒野生稻的遗传多样性[J].植物学报,2002,42(7):741-750.
[18] Manimekalair R, Nagarajan P. Assessing genetic relationships among coconut (Cocos nucifera L.) accessions using inter simple sequence repeat markers[J]. Scientia Horticulturae,2006,108(1): 49-54.
[19] Pereral L, Russell J R, Provan J, et al. Evaluating genetic relationships between indigenous coconut（Cocos nucifera L.）accessions from Sri Lanka by means of AFLP proling[J]. Theor Appl Genet,1998,96:545-550.