Abstract: On six different wheat varieties, respectively vernalization, devernalization and low temperature processing, by measuring peroxidase (POD) changes, to research and analysis of different varieties of wheat hardiness changes. The results showed that, in different treatment conditions were the first of its POD activity increased, reaching peak and then begin to decline. The different treatment of different wheat varieties under different time peak rising. Different wheat varieties activity trends also had significant differences. Vernalization ‘Aikang 58’, ‘Bama 196’ and ‘Zhoumai 22’ were in the process of vernalization 10 days, POD appeared strongest activity, while ‘Zhoumai 18’, ‘Bainong 207’ and ‘Bainong 69’ were in the process of vernalization for 20 days POD appeared strongest activity. POD vernalization off peak appeared at 20 days, however, POD activity increased more pronounced than that of vernalization. ‘Bama 196’ and ‘Zhoumai 18’ for 10 days in a peak, ‘Bainong 207’ and ‘Aikang 58’ for 20 days at the peak. But ‘Zhoumai 22’ declined, ‘Bainong 69’ for 30 days at a peak, the effect was not obvious. Description POD protective enzymes and cold resistance are closely related, and most varieties after a certain time of vernalization its hardiness improved significantly.