Immunostimulatory Effects of LPS and β-glucan on Subunit Vaccine of Edwardsiella tarda

doi:10.11924/j.issn.1000-6850.2013-1983

Abstract

Abstract: In order to investigate the immunostimulatory effects of LPS and β-glucan, an highly effective vaccine candidate for Edwardsiella tarda known as glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was recombinantly expressed in Escherichia coli, which was injected into Japanese flounder alone or containing LPS and β-glucan. After immunization, the antibody titer, expression of immune-related genes and immunological protection were detected. ELISA analysis of sera showed that the fish immunized with rGAPDH containing LPS and β-glucan had significant higher antibody titers than rGAPDH immunization group at day 14 and 30， which reached a value of 211.76 at day 30 after immunization. Simultaneously, the expression of 6 immune-related genes involved in innate and acquired responses, including IL-1β, IL-6, MHC-IIα, IgM, TCRα and C-type lysozyme were analyzed by quantitative PCR. The transcript levels of all selected genes displayed up-regulation after immunization, and immunization with rGAPDH containing LPS and β-glucan could induce significant higher levels than that induced only by rGAPDH. Moreover, to investigate the protection against infection, challenge was performed at day 30 after immunization. Fish immunized with rGAPDH containing LPS and β-glucan exhibited the highest relative percentage survival (RPS) of 67 % compared to the other groups. These results indicated that LPS and β-glucan employed as immunostimulatory molecules could significantly trigger the innate and acquired immune responses in Japanese flounder.

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