Abstract: In order to provide fertilizer application on a large scale for‘Zhenmai168’and the same types of wheat,‘Zhenmai168’, a strong gluten wheat as material. This experiment treatment applied in two factor randomized block design. The objective of the paper is to study high yield and grain quality characters by nitrogen topdressing. Results indicated that, after applied the same amount of base fertilizer, in certain extent, yield, content of protein, wet gluten content and dough stability time are just like a parabola. With the equal N application rate, there was a significant effect of yield-increasing due to nitrogen topdressing for‘Zhenmai168’at the jointing stage, and the average yield increased by 19.4% over the control. Nitrogen fertilizer with a rate of 112.5 kg/hm² resulted in the most significant yield improvement, with growth mass of 1853.7 kg/hm² than the control as high as 34.3% , meanwhile, crude protein content, wet gluten content, dough stability time, sedimentation value and unit weight. In a word, under this ecological conditions in south areas of Jiangsu Province, nitrogen fertilizer application of 112.5 kg/hm² during the jointing stage will be nitrogen strategies technique with high yield and efficiency.