Abstract: Effect of uniconazole on morphological traits, yield, crude fat content, protein content, soluble sugar content in kernel of different peanut variety was studied, and using the ‘Huayu 19’ and the ‘Huayu 23’ as materials. Uniconazole could increase diameter of main stem and promote growth of roots, and increase root dry weight and fruit dry weight per plant. Growth of the plant height could be delayed by foliar spray of uniconazole during flowering stage，and the greater of uniconazole concentration, the more obvious of effects. The effects were the greatest with 160 mg/L uniconazole. The root shoot ratio and economic coefficient could be improved by uniconazole treatment. The weight of pods per plant, kernel per plant, 100-pod weight and 100-kernel weight were significantly increased by uniconazole treatment. The root shoot ratio increased from 0.8904 to 1.2015, the economic coefficient increased from 46.57% to 53.46%, the yield increased from 18.09% to 69.10%. The seed fatty accumulation and the protein accumulation and the accumulation of soluble sugars can be promoted by uniconazole.