Abstract: To assess the effects of different N application levels on nitrogen metabolism and growing development of high quality edible-type and small grain peanut (‘Ganhua 7 hao’), this paper researched the responses of nitrogen metabolism, nodule growth and yield under N application levels (N0, 0 kg/hm²; N75, 75 kg/hm²; N150, 150 kg/hm²; N225, 225 kg/hm²; N300, 300 kg/hm² and N375, 375 kg/hm²) by combining of field experiment and laboratory analysis. The results showed that soluble protein contents, nitrate reductase activity and glutamine synthetase activity of leaf related to nitrogen metabolism, nodule growth and grain yield were improved first and reduced afterwards with the increasing amount of the N- fertilization increasing at each growth period. Leaf nitrogen metabolism and yield of peanut were the best and nodule number and size were more under N225 treatment. Nitrogen use, nodule growth and yield of‘Ganhua 7 hao’were better at 150-225 kg/hm² N application levels.