Abstract: To evaluate the genetic diversity and relationship among 25 clones of Jatropha curcas, the SRAP marker technology was used to analyze the difference of genomic DNA of above materials. The results showed that, 31 highly polymorphic and stable primer pairs were selected from 41 pairs of SRAP primer. With each primer combination generating 10-28 bands, 142 polymorphic bands were detected, accounting for 25.4% of the total. The similarity coefficient of the 25 clones was 0.409-0.951, with the average similarity coefficient of 0.714. In all clones, number 3 and number 7 had the greatest similarity coefficient, while number 6 and number 11 had the minimum one. 25 Jatropha germplasms were divided into 5 groups on the similarity level of 0.72. 7 clones had their respective specific bands, which could distinguish them from others. It is demonstrated that SRAP markers can be used for variety identification and population genetic construction of Jatropha. The conclusion is that, although the genetic diversity of 25 clones is not rich, they are different from each other on the molecular levels.