Abstract: Biological control is an important way of prevention of plant pathology. In order to study the appliaction of biological control measures in the process of wheat seed storage, the biocontrol Bacillus subtilis strain JGA2(-5)27-2 was used through this researching to study the characteristic as living preparation agent. B. subtilis strain JGA2(-5)27-2 not only had the ability to produce plant hormones cytokinins, but also had a wide spectrum of anti pathogenic fungi. Results of wheat germination test showed that the living preparation agent of B. subtilis strain JGA2(-5)27-2 had increased the wheat germination rate by 10.0% compared with CK treatment. Fungal infection test also showed that the living preparation agent of B. subtilis strain JGA2(-5)27-2 had the ability to prevent fungal infection in some extent. So the living preparation agent of B. subtilis strain JGA2(-5)27-2 has the potential to be used in the seed storage.