Abstract: To provide scientific support for the recycle of biogas slurry, we investigated the effects of biogas slurry on the grain quality, grain yield of wheat and soil properties under the field experimental conditions, using‘Yangmai 16’as the experiment material. The results showed that biogas slurry could increase tiller number, percentage of earbearing tiller, compared with no biogas treatment (CK). In addition, the contents of chlorophyll SPAD value, leaf area index, and dry matter accumulation had a corresponding increase with the increase of the application rates of biogas slurry. With application rates of biogas slurry increasing, soil bulk density had a decline tendency but porosity was increased. Among all treatments, the soil bulk density of TR5 (biogas slurry of 60000 kg/hm² + Urea of 150 kg/hm²) and TR6 (biogas slurry of 75000 kg/hm² + Urea of 150 kg/hm²) had reached 1.0 g/cm3. The biogas slurry could significantly increase soil nitrogen, phosphorus and potassium content, with the largest rate of rise 15.29% for total nitrogen, 34.55% for available phosphorus, and 16.18% for slowly available K, respectively. Biogas fertilizer with 60000 kg/hm² had the highest yield, reaching 6525.00 kg/hm², 6.5% higher than the yield of control treatment. The application rates of biogas slurry ranged from 60000 kg/hm² to 75000 kg/hm² had the best value for spreading.