Abstract: The study aimed at the influence of different seedling raising substrates on mechanized rice production, to find out the raising substrates with great extension value. First, the experiment of gradient dosages was carried out to investigate the suitable dosage of substrate mate, using Oryza sativa cv. ‘Jinzao 47’. Then the influence of four types of substrates including inorganic substrate, substrate mate, seedling-strengthening agent (SSA) and soil (CK) on mechanical transplanting seedling quality and yield were compared, using Oryza sativa cv. ‘Jinzao 09’ and ‘Yongyou 9’. Results showed that, the suitable dosage of substrate mate was substrate mate:soil =1:3. Result of substrate comparison showed three indexes of seedlings raised on inorganic substrate, substrate mate and SSA were higher than CK, including leaf age, basal stem width and dry matter weight, all of which for two varieties were highest on inorganic substrate. In addition, effective panicle number and yield were also higher than CK, while the highest yields of ‘Jinzao 09’ was on inorganic substrate, increasing by 2.88%, and ‘Yongyou 9’ was on substrate mate, increasing by 8.86%. The three substrates were all beneficial to nurse strong seedling and improve yield, while among these inorganic substrate and substrate were with better functions, thus were with great extension values at rice production.