Abstract: To study on relationship between chlorophyll contention of top-three leaves at grain filling stage and grain yield and quality traits in wheat, the chlorophyll contention of top-three leaves, spike grain number, 1000-grain weight, spike grain weight, protein content and wet gluten content were measured with 49 wheat genotypes as the experimental material. The results showed that: the difference was significant between genotypes, top-three leaves, different grain filling stages. The difference of spike grain number, 1000-grain weight, spike grain weight, protein content and wet gluten content between genotypes were significant; chlorophyll content of top-three leaves and spike grain number, 1000-grain weight, spike grain weight, protein content and wet gluten content were positive correlation, it was significant that the correlation between spike grain number and the second top leave on May 10th, the second top leave and the third top leave on May 15th and on May 19th, it was significant that correlation of 1000-grain weight and flag leave on May 19th, top-three leaves (flag leave, the second top leave and the third top leave) on May 24th, the correlation between spike grain number and chlorophyll content of the third top leave on May 10th, the second top leave and the third leave on May 15th, the top-three leaves (flag leave, the second top leave and the third leave) on May 19th, flag leave and the second top leave on May 24th were significant. It were significant that correlation of chlorophyll content of different grain filling stages expert flag leave on May 10th and protein content and wet gluten content. There was important influence on grain yield and quality traits about chlorophyll content of the top-three leaves in grain filling stage.