Abstract: To study the effects of different storage methods and time on content of nutrients in biogas slurry of straw, two storage methods were carried out on biogas slurry between open storage and airtight storage conditions at normal atmospheric temperature. The content of N, P, K，and organic matter in biogas slurry of straw were determined in different storage time. The results showed that: during the process of biogas slurry storage, little change occurred in the content of the organic matter while the total content of N, P, K was significantly declined; up to 50 days, the total content of N, P, K reduced to nearly 80%~90%; Because the content of N, P, K in biogas slurry reduced less in airtight storage conditions so that a better result was found on airtight storage methods than open storage methods in fertilizer field of biogas slurry of straw.