Studies on Activities of Digestive Enzymes and Nonspecific Immune Enzymes of Parapenaeopsis hardwickii at Different Growth Stages

doi:10.11924/j.issn.1000-6850.2013-2567

Abstract

Abstract: The aim of this trial was to investigate nutritional requirement and nonspecific immunity of Parapenaeopsis hardwickii at different growth stage. Large sums of samples collected at different times (day 0, 10, 17, 21, 34, 63, 77, 90 after culture) were used to evaluate the activities of digestive enzymes (acid protease, alkaline protease, amylase, lipase) and nonspecific immune enymes (acid phosphatase, alkaline phosphatase, superoxide dismutase) of Parapenaeopsis hardwickii in hepatopancreas.The results showed that the activities of digestive enzymes and nonspecific immune enzymes in hepatopancreas of Parapenaeopsis hardwickii changed significantly with the shrimp growth. Alkaline protease activity reached to the highest peak at 10 day and the minimum and maximum were found at 17 and 90 days. Acid protease activity was not detected at 0 day and the highest activity was found at 63 day. The activities of amylase and lipase fluctuated in wave-like curves. Amylase activity reached to the maximum level at 0 day, and two peaks were detected at 34 and 77 days, respectively. Lipase activity reached to the two peaks at 21 and 77 day and the lowest level was at 90 day. Both of acid phosphatase and superoxide dismutase activities reached to the low at 21 day, after that, they began to dropped at 77 and 63 day, respectively. Alkaline phosphatase activity reached to the lowest and highest levels at 17 and 63 day, respectively.The nonspecific immunoenzyme activities of Parapenaeopsis harbwickii could be enhanced by adding adequate immunostimulants to bait before 21 day (body length<36 mm) and after 63 day (body length>57.85mm) of the culture period.

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