Abstract: In order to explore biological control strains for effectively preventing and controlling plant diseases, Bionectria ochroleuca was introduced and its antagonistic effects on several plant pathogenic fungi were investigated, aiming at laying a theoretical foundation for its widespread application. In our study, the introduced fungal B. ochroleuca exhibited a varying degree of inhibition on plant pathogenic fungi including Alternaria solani, Botrytis cimerca, Fusarium oxysporum. sp cucumebrium Owen, Fusarium graminearum Sch1. f. sp vasinfectum (Atk.) Snyd & Hans., Colletotrichum capsici, Fusrium moniliforme, Fusarium graminearum, and Cercosporidium sofinum, with average width of inhibition zone was at 3.5~14.4 mm. Besides, the fermentation product of B. ochroleuca also showed good inhibitory effects on hyphal growth and spore germination of the pathogens, implying B. ochroleuca could secrete some extracellular antibacterial substances. However, its fermentation product displayed significant difference in inhibitory effects regarding to different strains, especially, showed the best inhibitory effects on F. oxysporum. sp cucumebrium Owen, with hyphal growth inhibition rate up to 73.3% and relative inhibition rate of spore germination was 78.2%, and in addition, it could lead to the distortion of germ tube which was used for spore germination. Furthermore, the fermentation product of B. ochroleuca could dramatically decrease the activity of intracellular protective enzymes as SOD and CAT (P<0.05) in pathogenic fungal hyphae. After treated for 21 hours, the SOD activity within the hyphae of F. oxysporum. sp cucumebrium was just 28.8% of the control, and CAT was 34.8% of the control. The above results showed that the introduced fungus B. ochroleuca had a certain application potential of being used as a biological control strain for plant pathogenic fungi.