Cloning and Bioinformatics Analysis of prcA in Lactobacillus paracasei

doi:10.11924/j.issn.1000-6850.2013-2611

Abstract

Abstract: The aims were to investigate the structure and function of prcA gene in Lactobacillus paracasei, and find out the relationship between prcA gene and autoinducing peptide gene of putative quorum sensing related genes in Lactobacillus paracasei. prcA gene was cloned by polymerase chain reaction (PCR) and predicted by bioinformatic methods. The prcA was 416 bp, containing a 138 bp open reading frame (ORF), which encoded 45 amino acids, while the estimated molecular weight and isoelectric point of the putative protein were 5326.83 Da and 4.85, it was a stability protein without membrane- spanning domain, alpha helix, random coil and extended strand were major structural elements of polypeptide chain, subcellular localization result indicated that it was located outside the cell, prcA is the putative quorum sensing related gene in Lactobacillus paracasei encoding autoinducing peptide, which regulates bacteriocin production.

ping jingge. Cloning and Bioinformatics Analysis of prcA in Lactobacillus paracasei[J]. Chinese Agricultural Science Bulletin, 2014, 30(18): 259-263.

References

[1] Spoering A L, Gilmore M S. Quorum sensing and DNA release in bacterial biofilms[J].Current Opinion in Microbiology,2006,9(2): 133-137.
[2] Kleerebezem M, Bongers B, Rutten G, et al. Autoregulation of subtilin biosynthesis in Bacillus subtilis: the role of the spa-box in subtilin-responsive promoters[J].Peptides,2004,25(9):1415-1424.
[3] Laverty G, Gorman S P, Gilmore B F. Biomolecular mechanisms of staphylococcal biofilm formation[J].Future microbiology,2013,8(4): 509-524.
[4] de Vos W M, Kleerebezem M, Kuipers O P. Expression systems for industrial Gram- positive bacteria with low guanine and cytosine content[J].Current Opinion in Biotechnology,1997,8(5):547-553.
[5] West A H, Stock A M. Histidine kinases and response regulator proteins in two- component signalling systems[J].Trends in Biochemical Sciences,2001,26(6):369-376.
[6] Schuster M, Greenberg E P. A network of networks: Quorumsensing gene regulation in Pseudomonas aeruginosa[J]. International Journal of Medical Microbiology,2006,296(2):73-81.
[7] Alleson D, Cotter P D, Ross R P, et al. Bacteriocin Production: a Probiotic Trait[J].Applied and Environmental Microbiology,2012,78 (1):1-6.
[8] Barnard A M L, Bowden S D, Burr T, et al. Quorum sensing, virulence and secondary metabolite production in plant soft-rotting bacteria[J].Philosophical Transactions of the Royal Society B: Biological Sciences,2007,362(1483):1165-1183.
[9] Jayaraman A, Wood T K. Bacterial quorum sensing: signals, circuits, and implications for biofilms and disease[J].Annual Reviews Biomedical Engineering,2008,10:145-167.
[10] Ge J P, Ping W X, Song G, et al. Paracin 1.7, a bacteriocin produced by Lactobacillus paracasei HD1.7 isolated from Chinese cabbage sauerkraut, a traditional Chinese fermented vegetable food[J].Acta Microbiologica Sinica,2009,49(5):609-616.
[11] 房保柱.副干酪乳杆菌HD1.7的群体感应现象研究及与拮抗菌的生态学关系[D].哈尔滨:黑龙江大学,2012.
[12] Nakayama J, Akkermans A D L, Willem M D V. High-throughput PCR screening of genes for three- component regulatory system putatively involved in quorum- sensing from low G + C grampositive bacteria[J].Bioscience Biotechnology & Biochemistry,2003, 67(3):480-489.
[13] 张岚.副干酪乳杆菌HD1.7prcK基因敲除载体构建及其电转化条件初步确立[D].哈尔滨:黑龙江大学,2008.
[14] 赵晓龙. L.paracasei HD1.7 prcR定点突变及pMG36e对该菌的转化[D].哈尔滨:黑龙江大学,2008.
[15] Gray B, Hall P, Gresham H. Targeting agr- and agr- Like Quorum Sensing Systems for Development of Common Therapeutics to Treat Multiple Gram-Positive Bacterial Infections[J].Sensors,2013, 13(4):5130-5166.
[16] Dunny G M, Leonard B A. Cell- cell communication in Grampositive bacteria[J].Annual Review of Microbiology,1997,51(1): 527-564.
[17] Nes I F, Diep D B, H?varstein L S, et al. Biosynthesis of bacteriocins in lactic acid bacteria[J].Antonie van Leeuwenhoek, 1996,70(2-4):113-128.
[18] Eijsink V G H, Brurberg M B, Middelhoven P J, et al. Induction of bacteriocin prodcution in Lactobacillus sakei by a secreted peptide [J].Journal of Bacteriology,1996,178(8):2232-2237.