Abstract: In order to achieve the degradation of pesticide residues safely and scientifically, roast eels were irradiated by 60Co-γ ray at different radiation doses (0kGy, 1kGy, 3kGy, 5kGy, 7kGy, 9kGy). The effects of radiation dose on the degradation rate of the ofloxacin, ciprofloxacin, enrofloxacin, difloxacin and leucocrystal violet were studied. The results showed that irradiation can degrade the ofloxacin, ciprofloxacin, enrofloxacin, difloxacin and leucocrystal violet, respectively. When the radiation dose was 5kGy, the degrading effects were significant. And the degradation rate of ofloxacin, ciprofloxacin, enrofloxacin, difloxacin and leucocrystal violet were 37.89%，41.77%，41.76%，57.34%，32.89%, respectively. With the increase of radiation dose, the degradation rate of ofloxacin, ciprofloxacin, enrofloxacin, difloxacin and leucocrystal violet went up.