Abstract: In order to improve the detection sensitivity of saponins, a kind of high performance liquid chromatography(HPLC) coupled with evaporative light scattering detector(ELSD) was established for the determination of hederacoside C and α-hederin in Hedera nepalensis var. sinensis. A welchrom column (250 mm×4.6 mm, 5 μm) was used with the mobile phase of water and acetonitrile solution. The flow rate was at 1.0 mL/min. This method showed good linearity for both hederacoside C (ranging from 0.19188 to 2.3985 μg) and α-hederin (ranging from 0.15615 to 4.6844 μg). The results demonstrated that this method was precise, accurate and can be used for the determination of hederacoside C and α-hederin .