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Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (13): 25-28.doi: 10.11924/j.issn.1000-6850.2013-2781

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SSR Analysis of Genetic Diversity of Ping’ou Hybrid Hazelnut

  

  • Received:2013-10-25 Revised:2014-01-02 Online:2014-05-05 Published:2014-05-05

Abstract: The analysis of genetic relationship of Ping’ou hybrid hazelnut laid the foundation for the genetic mapping and the breeding of choiceness gene. 21 main cultivars were selected for the experiment. After DNA extraction, SSR molecular marker and NTSYSpc (version 2.11 a) analysis of the results, constructed a dendrogram. 6 primer pairs of Corylus avellana were selected for the detection of the tested materials. 71 genetic loci were detected, which were polymorphic loci in all. The percentage of polymorphic bands was 100%. The SSR-derived genetic similarity was 0.34-0.82, with an average of 0.55. All the samples were divided into 4 groups when the threshold value was 0.74. C1, C13 and C2 constituted the groupⅠ; C6 and C15 constituted the groupⅡ; C7, C9, C14, C16 and C10 constituted the groupⅣ. The others constituted the groupⅢ. There were genetically abundant diversities among Ping’ou hybrid hazelnut. The genetic relationships of the main cultivars were complex. Analysis system established in this study could offer reference for other relative researches.