Abstract: Experiment chose seed development Limoniumau- reum no vaccine hypocotyl cotyledon stem section as explant materials, study the culture of different explant technique, the results showed that: the suitable medium auxin 2,4- D on adventitious bud induction had an obvious role in promoting, when its concentration was 1.5 mg/L induction rate was highest, cotyledon was good explant of adventitious bud induction, the optimal culture medium for MS+2,4-D 1.5 mg/L+6-BA 2.0 mg/L+NAA 1.0 mg/L. Limoniumaureum optimal proliferation medium was MS+6-BA 1.0 mg/L+ NAA 1.0 mg/L, and took the form of multiple shoot clumps proliferation, The optimum rooting medium was 1/2 MS+ KT 1.0 mg/L+ IBA 1.0 mg/L.