Abstract: In order to study on application of novel radiation (LED) resource in Spathiphyllum floribundum in vitro, the purpose of this experiment was to get high-quality in vitro and large-scale production, adopted the ultra bright red (R) & blue (B) LED as light emitting materials, to use bright leaves of Spathiphyllum floribundum the experiment plant material to explore the effectiveness of light-emitting diodes and the cold cathode fluorescent lamp in the production for transplanted Spathiphyllum floribundum jamesonii in vitro, to discuss the best light quality for transplanted Spathiphyllum floribundum in vitro large-scale production and to discuss the perspective of novel radiation resource LED in Spathiphyllum floribundum in vitro production. The results showed that：the stem length reaches the maximum in the LED of the red/blue ratio of 5:5. The leaf blade and blade width reaches the maximum in the LED of the red/blue ratio of 8:2, and there was significant difference between them and CK. The roots reached the maximum in the LED of the red/blue ratio of 8:2. The root length reached the maximum in the LED of the red/blue ratio of 5:5. The fresh weight and dry weight all reached the maximum in the LED of the red/blue ratio of 8:2. Only in the LED of the red/blue ratio of 5:5, the chlorophyll content was higher than CK. The dry matter rate reached the maximum in the LED of the red/blue ratio of 7:3. But the root vigor reached the maximum in the LED of the red/blue ratio of 8:2. To sum up, the transplanted Spathiphyllum floribundum in the LED of the red/blue ratio of 8:2 was under the best growth condition.