Abstract: In order to investigate the effects of differences which the content of sugar and organic acids in fluecured tobacco in different areas of Hunan on aromatic style, using gas chromatography, the difference and its pertinence analysis of the content of sugar and organic acids in middle leaf of flue-cured tobacco at the Hunan different areas were studied. The different areas included Fenghuang, Chenzhou, Cili and Shaoyang. Results indicated that: The content of soluble total sugar and the return to original state sugar in Fenghuang and Shaoyang were significantly richer than Cili and Chenzhou, but the non volatile acid was quite the opposite. In 4 flue-cured tobacco regions, the content of total non-volatile acid, volatile acid and organic acids in Cili were highest, followed by Chenzhou, Fenghuang was the lowest, but Fenghuang contained the highest total senior fatty acids, and with the other areas existed higher difference. The majority content of non-volatile acids exited remark difference except malonic acid. In 4 flue-cured tobacco regions, the content of isobutyric acid, valeric acid and 3- methyl pentanoic acid existed remark difference but butyric acid was not notable. The others existed difference but the difference of four productions existed no laws. The content of senior fatty acids in three tobacco production regions of Cili, Chenzhou and Fenghuang existed small difference, but with the Shaoyang existed higher difference except myristic acid.