Abstract: In order to reveal the characteristics of humus compounds and organic nitrogen compounds and their relationships with altitude in tobacco planting soil of Zhangjiajie, 25 soil samples were collected from four pathway located by GPS technology in different altitude and their humus compounds and organic nitrogen compounds were analyzed. The results showed that the average amount of soil humus, humus aicd, humic aicd and soil organic matter activity reached the peak in the altitude of 601-800 meter, the average amount of fulvic acid and humin were much high in the same altitude. The compositions of acid organic nitrogen represented clear regular as the altitude change, ammonium nitrogen, amino acid nitrogen and unknown nitrogen all increased and then decreased with the rising of altitude, total acid hydrolysable nitrogen, acid hydrolysable ammonium nitrogen, acid hydrolysable amino nitrogen and unknown acid hydrolysable nitrogen all got the high level in the altitude of 400-600 m, the comprehensive nitrogen supplying capacity were best in this altitude. Soil humus compounds and organic nitrogen compounds showed significant correlation relationship, the relationships between amino acid nitrogen and humus aicd, humic aicd, humin and the relationships between amino sugar nitrogen and humus aicd, fulvic acid, humin were all highly significant.