Abstract: For the purpose of part of the potato varieties in Northeast region identification at the molecular level .18 pairs of SSR (Sinple Sequence Repeats) primer markers were employed to analysis of genetic and construction of fingerprinting for 18 potato varieties .The results show: 156 alleles loci were amplified including 146 polymorphic alleles, and the ratio of polymorphism reached 92.4%. Fragment amplification products in 100~800bp.The fingerprinting of 17 potato varieties was constructed by 7 pairs of primers，STM1021、STI051、S170、S7、STI030、STPATP1 and STM2023,All the potato varieties were univocally identified using SSR primer S7. The UPGMA cluster analysis showed that , At the genetic coefficient of 0.63，14 potato varieties were divided into two groups．The cluster analysis result was accordance with the genetic relationships. The genetic diversity of the tested potato was relatively narrow .