Abstract: Purity identification, molecular ID and analysis of genetic relation of cultivars were completed for test cultivar in the national north spring soybean cultivar authorize. These work had great contribution to the cultivar breeding and spread. 13 SSR markers which distributed in 8 linkage groups of soybean were used to identify 94 cultivars belong to the tested varieties of national north spring soybean cultivar authorize in 2012. The results showed that the purity of these varieties ranged from 53.85% to 100%, and averaged 99.02%. Five SSR markers including Satt231, Satt288, Satt160, Satt193 and Sat-092, were used to identify the 94 soybean varieties, then a unique molecule ID was obtained for each variety. The genetic similar coefficient of 94 varieties ranged from 0 to 0.846, the average value was 0.2783, and the result indicated the genetic difference was huge in the 94 tested varieties. SSR analysis method was an effective method to identify the purity, construct molecular ID and complete analysis of genetic relation for the tested varieties.