Induction of Adventitious Root in Pinus tabulaeformis

doi:10.11924/j.issn.1000-6850.2014-0259

Abstract

Abstract: To establish a model system with high frequency adventitious rooting in Pinus tabulaeformis, adventitious buds and hypocotyl cuttings from this species were used to induce adventitious roots. Then, effect of cotyledon, age of seedlings, plant growth regulators and treatment methods on the induction of adventitious roots from hypocotyls were studied. The results showed that hypocotyl cuttings have more stronger potential to induce adventitious root than adventitiou buds. And suitable medium was 1/2 DCR medium containing 20 g/L sucrose and 6 g/L agar with pH 5.8. When cultured the hypocotyl cuttings from 2-week old seedlings on this medium with 4 mg/L IBA for 15 days, then turned the culture to the same medium without IBA, the frenquency of adventitious rooting high to 96.67% was obtained. This provided a feasible experiment system for the further research on mechanism about adventitious root formation in Pinus tabulaeformis.

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