Abstract: In order to find out the best method and regression parameter for leaf area determination of Macadamia spp., three kinds of leaf area measurement methods were compared, the relationship between different leaf parameters and leaf area were regressively analyzed. The results showed that there was no significant difference among the leaf area values measured by three different methods of the same variety, the correlation coefficient of leaf area values measured by three determination methods was more than 0.999, all the three leaf area measurement methods, including criss- cross method, cut- weight method and scanning method, could be used as a reliable method of Macadamia spp. leaf area determination. The regression coefficient of different leaf parameters and leaf area of different varieties were different, the product of leaf length and width was the best parameter which could reflect the actual leaf area of Macadamia spp.. The study provided an important reference for physiological research and production practice of Macadamia spp..